Part:BBa_K3590038

Lysozyme derived from Bacillis licheniformis fused with a dockerin derived from Clostridium thermoce

This synthetic construct was designed to lyse cyanobacterial cell walls while being displayed on ScafL.

At the 5' end of LysBL-DocCt there is the Pveg promoter followed by RBS R1. The CDS encodes several functional modules - SacB signal sequence for extracellular transport, His-tag for Western blot detection followed by lysozyme derived from Bacillus licheniformis (LysBL). It has been shown that LysBL does not have a negative effect on B. subtilis but its activity against cyanobacteria is, to our knowledge, still unknown. The LysBL module is followed by a linker connecting it to the dockerin domain of Clostridium thermocellum. This dockerin interacts exclusively with a cohesin domain originating from the same species so it ensures the attachment of this whole fusion protein to ScafL. At the 3' end of the CDS, there is a STOP codon.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]